6W66
The structure of the F64A, S172A mutant Keap1-BTB domain in complex with SKP1-FBXL17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.11583 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 183.665, 183.665, 55.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.530 - 3.210 |
| R-factor | 0.2535 |
| Rwork | 0.252 |
| R-free | 0.28960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cxi 2ass 1fqv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.418 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 159.060 | 159.060 | 3.430 |
| High resolution limit [Å] | 3.210 | 9.070 | 3.210 |
| Rmerge | 0.289 | 0.033 | 4.145 |
| Rmeas | 0.304 | 0.035 | 4.363 |
| Rpim | 0.093 | 0.011 | 1.344 |
| Total number of observations | 8425 | 32010 | |
| Number of reflections | 17752 | 846 | 3139 |
| <I/σ(I)> | 7.6 | 52.5 | 0.7 |
| Completeness [%] | 99.6 | 99.8 | 98 |
| Redundancy | 10.6 | 10 | 10.2 |
| CC(1/2) | 0.998 | 1.000 | 0.452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | well solution 37.5 ul of 50 mM MgCl2, 100 mM HEPES pH 7.5, 30% (v/v) PEG MME 550 (E7 Hampton Index) + 12.5ul H2O mixed 1:1 with complex at 15mg/ml in 150 mM NaCl, 25 mM Tris/HCl pH 8.0 |
