6W4I

APE1 Y269A product complex with abasic DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-22
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	45.381, 150.233, 156.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.580 - 2.200
R-factor	0.1867
Rwork	0.185
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dff
RMSD bond length	0.008
RMSD bond angle	1.028
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.580	2.240
High resolution limit [Å]	2.200	2.200
Rmeas	0.073	0.440
Number of reflections	91210	2649
<I/σ(I)>	20.6
Completeness [%]	99.5	96.9
Redundancy	5.9	3.5
CC(1/2)		0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	24% PEG 3350, 0.1 M Bis Tris Propane (pH 6.5), 0.2 M Sodium Fluoride