6VZW

TTLL6 bound to the initiation analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	75.254, 110.585, 173.579
Unit cell angles	90.00, 90.14, 90.00

Refinement procedure

Resolution	19.920 - 2.500
R-factor	0.2184
Rwork	0.217
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ylr
RMSD bond length	0.002
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.920	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.045	0.333
Rmeas	0.064	0.471
Rpim	0.045	0.332
Number of reflections	191456	19357
<I/σ(I)>	7.35	1.97
Completeness [%]	99.7	99.77
Redundancy	2	2
CC(1/2)	0.998	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	290	100 mM Sodium Citrate, pH 6.2, 200 mM MgCl2, 8-12% Peg 20000