6VZO

Crystal structure of human PPARgamma ligand binding domain (Protein delipidated by denature and refold)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	93.040, 62.100, 118.850
Unit cell angles	90.00, 102.22, 90.00

Refinement procedure

Resolution	58.079 - 2.270
R-factor	0.2202
Rwork	0.218
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1prg
RMSD bond length	0.008
RMSD bond angle	0.967
Data reduction software	DIALS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.080	2.351
High resolution limit [Å]	2.270	2.270
Rmerge	0.037	0.341
Rmeas	0.052
Rpim	0.037
Number of reflections	30849	2997
<I/σ(I)>	8.43	1.58
Completeness [%]	99.5
Redundancy	2	1.9
CC(1/2)	0.997	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.1M Tris, pH 7.4 0.8M SODIUM CITRATE