6VYJ

Human UHRF1 TTD domain in complex with a fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	83
Detector technology	PIXEL
Collection date	2018-02-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97741
Spacegroup name	P 21 21 21
Unit cell lengths	40.280, 55.350, 66.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.480 - 1.390
R-factor	0.199
Rwork	0.198
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qqd
RMSD bond length	0.008
RMSD bond angle	0.984
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.260	1.410
High resolution limit [Å]	1.390	1.390
Number of reflections	30553	1464
<I/σ(I)>	7
Completeness [%]	100.0
Redundancy	6
CC(1/2)	0.993	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM Bis-Tris, pH 5.75, 35 % w/v PEG 3350. The crystals were cryo-preserved by 25% glucose