6VRR

Crystal structure of a disease mutant of the Voltage-gated Sodium Channel Beta 2 subunit extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	40.271, 53.582, 59.319
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.760 - 1.450
R-factor	0.1516
Rwork	0.150
R-free	0.18270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5feb
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.300	1.530
High resolution limit [Å]	1.450	1.450
Rmeas	0.043	0.630
Number of reflections	43929	2827
<I/σ(I)>	24.4	2.75
Completeness [%]	99.9	99.6
Redundancy	6.9	6.54
CC(1/2)	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1 M HEPES (pH 7.0), 14% PEG 6000