6VRQ

Crystal structure of gl12A21 Fab in complex with anti-idiotypic iv12 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.174, 202.858, 72.341
Unit cell angles	90.00, 93.01, 90.00

Refinement procedure

Resolution	36.120 - 2.570
R-factor	0.1913
Rwork	0.189
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mft
RMSD bond length	0.006
RMSD bond angle	0.764
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.120	2.660
High resolution limit [Å]	2.570	2.574
Number of reflections	34290	2332
<I/σ(I)>	21.98
Completeness [%]	99.7
Redundancy	2.66
CC(1/2)	0.997	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M KCl 0.1M Tris pH 8 15%w/v PEG MME 2K