6VQM
Crystal Structure Analysis of human ACK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-02-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.638, 42.453, 92.014 |
| Unit cell angles | 90.00, 99.13, 90.00 |
Refinement procedure
| Resolution | 90.930 - 2.870 |
| R-factor | 0.2733 |
| Rwork | 0.270 |
| R-free | 0.32960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u46 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.496 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.930 | 3.110 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Rmeas | 0.104 | 0.528 |
| Number of reflections | 12433 | 2663 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 98.0 | 99.9 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG4000 |
