6VPQ

Crystal structure of the C-terminal domain of DENR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	64.670, 47.260, 95.500
Unit cell angles	90.00, 106.29, 90.00

Refinement procedure

Resolution	91.667 - 1.740
R-factor	0.1812
Rwork	0.178
R-free	0.24730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vyc
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.667	91.667	1.790
High resolution limit [Å]	1.740	7.780	1.740
Rmerge	0.105	0.030	6.596
Rmeas	0.121	0.035	7.505
Total number of observations	249082
Number of reflections	56630	665	4138
<I/σ(I)>	7.84	35.88	0.18
Completeness [%]	98.9	94.5	99.4
Redundancy	4.398	4.018	4.363
CC(1/2)	0.997	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	285	0.1 M CaCl2, 15% PEG 400, 0.1 M Sodium acetate, pH 5.0