6VPG

TPX2 residues 7-20 fused to Aurora A residues 116-389 in complex with AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	83.562, 83.562, 114.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.080 - 2.640
R-factor	0.2037
Rwork	0.199
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ol5
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.740
High resolution limit [Å]	2.640	5.700	2.650
Rmerge	0.074	0.032	0.904
Rmeas	0.083	0.036	1.015
Rpim	0.037	0.017	0.456
Number of reflections	12410	1353	1211
<I/σ(I)>	25.7
Completeness [%]	99.8	98.5	99.6
Redundancy	4.9	4.6	4.8
CC(1/2)		0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	298	0.1 M HEPES pH 7, 0.9 M KCl, 1.08 M ammonium sulfate, 12% glycerol