6VOU

Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-plazomicin and CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 32 2 1
Unit cell lengths	73.510, 73.510, 147.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.660 - 1.950
R-factor	0.1935
Rwork	0.192
R-free	0.22490
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.660	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.052
Rmeas	0.055
Rpim	0.018
Number of reflections	33925	3293
<I/σ(I)>	18.89
Completeness [%]	98.8
Redundancy	8.1
CC(1/2)	0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	acetyl CoA, plazomicin, LiCl, MPD