6VO5

Crystal structure of Human histone acetytransferas 1 (HAT1) in complex with isobutryl-COA and K12A mutant variant of histone H4

Replaces:  6UHD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 2
Unit cell lengths	116.726, 155.636, 53.475
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.450 - 1.600
R-factor	0.1721
Rwork	0.171
R-free	0.19760
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.406
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.620	48.620	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.059	0.025	1.066
Rmeas	0.062	0.027	1.129
Rpim	0.021	0.009	0.367
Total number of observations	1132308	7435	57634
Number of reflections	128467	903	6322
<I/σ(I)>	20.3	66.9	1.9
Completeness [%]	99.5	98.3	99.5
Redundancy	8.8	8.2	9.1
CC(1/2)	0.999	0.999	0.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	12% PEG 20K and 0.1M MES pH6.5