6VNP

Crystal structure of DPO4 extension past 8-oxoadenine (oxoA) and dT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	120
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	53.255, 98.984, 102.875
Unit cell angles	90.03, 89.99, 89.92

Refinement procedure

Resolution	47.300 - 2.420
Rwork	0.199
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jx4
RMSD bond length	0.010
RMSD bond angle	1.271
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.470
High resolution limit [Å]	2.420	2.420
Rpim	0.061	0.454
Number of reflections	78571	4003
<I/σ(I)>	8.1
Completeness [%]	97.6
Redundancy	1.9
CC(1/2)		0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM Tris 7.5 20% PEG3350 100 mM Magnesium Acetate