6VMK
Crystal structure of human Complement Factor D with anti-Factor D Fab 20D12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 263.704, 301.454, 458.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.933 - 3.010 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.21480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | prior |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.533 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12-2829_final) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 251.945 | 251.945 | 3.011 |
| High resolution limit [Å] | 3.001 | 13.927 | 3.001 |
| Rmerge | 0.142 | 0.033 | 0.886 |
| Rmeas | 0.154 | 0.036 | 0.959 |
| Rpim | 0.059 | 0.014 | 0.364 |
| Total number of observations | 2447451 | 24632 | 17503 |
| Number of reflections | 359723 | 3843 | 2573 |
| <I/σ(I)> | 12.9 | 36.2 | 2.3 |
| Completeness [%] | 99.7 | 98.9 | 70.7 |
| Redundancy | 6.8 | 6.4 | 6.8 |
| CC(1/2) | 0.996 | 0.999 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1 M sodium citrate pH 5.0, 8% PEG 8000 |
