6VME

Human ESCRT-I heterotetramer headpiece

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11583
Spacegroup name	P 61
Unit cell lengths	162.584, 162.584, 139.715
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	99.180 - 2.190
R-factor	0.1943
Rwork	0.193
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f66
RMSD bond length	0.008
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.180	2.269
High resolution limit [Å]	2.190	2.190
Rpim	0.042
Number of reflections	107460	10167
<I/σ(I)>	17.22
Completeness [%]	99.5	95.09
Redundancy	20
CC(1/2)	0.999	0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.15	2 % w/v gamma-Polyglutamic acid (gamma-PGA), 100 mM sodium formate, 100 mM sodium acetate pH 5.0