6VLD
Crystal structure of human alpha 1,6-fucosyltransferase, FUT8 bound to GDP and A2SGP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9536 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.307, 68.453, 249.983 |
| Unit cell angles | 90.00, 111.21, 90.00 |
Refinement procedure
| Resolution | 48.550 - 2.280 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2de0 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 2.320 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.103 | 1.517 |
| Rmeas | 0.114 | 1.676 |
| Rpim | 0.047 | 0.700 |
| Total number of observations | 849036 | 41177 |
| Number of reflections | 150224 | 7302 |
| <I/σ(I)> | 10.2 | 1.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.7 | 5.6 |
| CC(1/2) | 0.998 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 281 | 2 M NH4SO4, 0.2 M NaCl, 0.1 M sodium cacodylate, pH 6.5 |
