6VKM
Crystal Structure of Stabilized GP from Makona Variant of Ebola Virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-10-26 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | H 3 2 |
Unit cell lengths | 113.110, 113.110, 393.180 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.530 - 3.500 |
R-factor | 0.2849 |
Rwork | 0.284 |
R-free | 0.30310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jq3 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.530 | 47.530 | 3.830 |
High resolution limit [Å] | 3.500 | 8.570 | 3.500 |
Rmerge | 0.346 | 0.156 | 0.743 |
Rmeas | 0.391 | 0.174 | 0.838 |
Rpim | 0.177 | 0.076 | 0.378 |
Total number of observations | 45250 | 3273 | 10985 |
Number of reflections | 10491 | 727 | 2527 |
<I/σ(I)> | 3.4 | 6 | 2 |
Completeness [%] | 84.7 | 80.6 | 86.7 |
Redundancy | 4.3 | 4.5 | 4.3 |
CC(1/2) | 0.913 | 0.972 | 0.564 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 9.8% PEG 6000, 0.1 M Sodium citrate pH 5.2, 3% glycerol |