6VHQ
Crystal structure of Bacillus subtilis levansucrase (D86A/E342A) in complex with oligosaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.313, 78.665, 78.732 |
| Unit cell angles | 90.00, 93.94, 90.00 |
Refinement procedure
| Resolution | 35.205 - 2.047 |
| R-factor | 0.1704 |
| Rwork | 0.167 |
| R-free | 0.23480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyg |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.476 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.205 | 2.090 |
| High resolution limit [Å] | 2.047 | 2.047 |
| Rmerge | 0.048 | 0.526 |
| Number of reflections | 53175 | 5145 |
| <I/σ(I)> | 23.9 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6 | 291 | 30 % w/v PEG 2000 MME and 150 mM Potassium Bromide |
