6VGR
Crystal Structure of Human Dipeptidase 3 in Complex with Fab of SC-003
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Collection date | 2018-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 167.030, 167.030, 116.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.120 - 2.840 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vgo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.120 | 2.890 |
| High resolution limit [Å] | 2.840 | 2.840 |
| Rmeas | 0.091 | |
| Rpim | 0.356 | |
| Number of reflections | 42049 | 2173 |
| <I/σ(I)> | 20 | |
| Completeness [%] | 96.2 | |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.5, 10% w/v PEG4000 |
