6VG8
Crystal structure of the DNA binding domains of human FLI1 and Runx2 in complex with 16-mer DNA CAGAGGATGTGGCTTC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-10 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 104.427, 104.427, 326.382 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.210 - 4.310 |
| R-factor | 0.2736 |
| Rwork | 0.273 |
| R-free | 0.28270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jvt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.221 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.131 | 0.433 |
| Number of reflections | 16635 | 696 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 93.8 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 7% w/v PEG 4000, 50 mM Na Citrate, pH 5.6, 4% v/v isopropanol |
