6VFW

Crystal structure of human delta protocadherin 10 EC1-EC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	346.550, 73.193, 246.328
Unit cell angles	90.00, 132.06, 90.00

Refinement procedure

Resolution	19.990 - 3.600
R-factor	0.2773
Rwork	0.277
R-free	0.29480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vfq
RMSD bond length	0.012
RMSD bond angle	0.783
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.710
High resolution limit [Å]	3.600	3.600
Rmerge	0.123	4.649
Rmeas	0.146	5.476
Number of reflections	52814	4543
<I/σ(I)>	5.4	0.4
Completeness [%]	98.2	98.3
Redundancy	3.4	3.6
CC(1/2)	1.000	0.290

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	296	12% (w/v) PEG 8000, 20% (v/v) ethylene glycol, 0.1M Tris-bicine buffer pH8.5, 10% 0.02 M D-glucose, 0.02 M D-mannose, 0.02 M D-galactose, 0.02 M L-fucose, 0.02 M D-xylose, 0.02 M N-acetyl-D-glucosamine with no further cryoprotection