6VEQ
Con-Ins G1 in complex with the human insulin microreceptor in turn in complex with Fv 83-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953646 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 106.160, 227.120, 228.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.146 - 3.250 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oga |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.504 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13-2998_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.259 | 2.748 |
| Number of reflections | 43876 | 3751 |
| <I/σ(I)> | 6.44 | 0.59 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.8 | 6 |
| CC(1/2) | 0.992 | 0.163 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 1.7 M ammonium sulphate 50 mM MOPS-NaOH (pH 7.0) |
