6VE5

X-ray structure of human REV7 in complex with Shieldin3 (residues 41-74)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9794
Spacegroup name	P 32 2 1
Unit cell lengths	59.990, 59.990, 132.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.250 - 2.000
R-factor	0.2178
Rwork	0.215
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3abd
RMSD bond length	0.004
RMSD bond angle	0.652
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.250	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.083	1.193
Rmeas	0.085	1.222
Rpim	0.018	0.262
Number of reflections	19399	1337
<I/σ(I)>	40.89	4.11
Completeness [%]	98.4
Redundancy	22.6
CC(1/2)	0.999	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	288	Protein complex was in 5 mM HEPES, pH 7.4, 100 mM NaCl, 5 mM DDT at 25 mg/mL;Reservoir solution was 0.1 M MES monohydrate, pH 6.5, 1.4 M MgSO4.6H20;Cryoprotection was done in 50% PEG 400