6VE1

Crystal structure of endo-beta-N-acetylglucosaminidase H at high pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 2 21
Unit cell lengths	93.480, 99.480, 135.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.031 - 2.100
R-factor	0.2225
Rwork	0.221
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1edt
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.031	2.175
High resolution limit [Å]	2.100	2.100
Number of reflections	74108	7330
<I/σ(I)>	5.89
Completeness [%]	99.7
Redundancy	2
CC(1/2)	0.986	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	9	295	PEG20000, magnesium nitrate, TAPS, pH 9.0