6VDD

POL domain of Pol1 from M. smegmatis complex with DNA primer-template and dNTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	66.664, 233.805, 169.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.530 - 1.900
R-factor	0.1916
Rwork	0.191
R-free	0.23660
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.048
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rpim	0.033	0.414
Number of reflections	104295	5171
<I/σ(I)>	35.6
Completeness [%]	99.9
Redundancy	7.6
CC(1/2)	1.000	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M magnesium chloride, 0.1 M MES, 25% PEG4000