6VDC

POL domain of Pol1 from M. smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-31
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97918
Spacegroup name	C 2 2 21
Unit cell lengths	62.591, 150.951, 150.348
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.830 - 2.402
R-factor	0.2226
Rwork	0.219
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uqg
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rpim	0.030	0.312
Number of reflections	28069	1368
<I/σ(I)>	33.6
Completeness [%]	99.1
Redundancy	6.3
CC(1/2)	0.996	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium chloride, 20% PEG3350