4UQG

A new bio-isosteric base pair based on reversible bonding

Summary for 4UQG

• Entry DOI: 10.2210/pdb4uqg/pdb
• Related PRD ID: PRD_900003
• Descriptor: DNA POLYMERASE, 5'-D(*GP*AP*CP*C T0TP*TP*CP*CP*CP*TP)-3', 5'-D(*AP*GP*GP*GP*A SAYP*GP*GP*TP*CP)-3', ... (6 entities in total)
• Functional Keywords: transferase-dna complex, unnatural base pair, transferase/dna
• Biological source: GEOBACILLUS STEAROTHERMOPHILUS; More
• Total number of polymer chains: 3
• Total formula weight: 73021.53

Authors

Tomas-Gamasa, M.,Serdjukov, S.,Su, M.,Mueller, M.,Carell, T. (deposition date: 2014-06-23, release date: 2014-12-03, Last modification date: 2024-01-10)

Primary citation

Tomas-Gamasa, M.,Serdjukow, S.,Su, M.,Muller, M.,Carell, T.
"Post-it" type connected DNA created with a reversible covalent cross-link.
Angew. Chem. Int. Ed. Engl., 54:796-800, 2015

PubMed Abstract: We report the development of a new heterobase that is held together through reversible bonding. The so-formed cross-link adds strong stabilization to the DNA duplex. Despite this, the cross-link opens and closes through reversible imine bonding. Moreover, even enzymatic incorporation of the cross-link is possible. The new principle can be used to stabilize DNA duplexes and nanostructures that otherwise require high salt concentrations, which may hinder biological applications.

PubMed: 25446281
DOI: 10.1002/anie.201407854

Experimental method

X-RAY DIFFRACTION (2 Å)