6VCS
SRA domain of UHRF1 in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-23 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.995, 113.809, 133.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.600 - 1.700 |
| R-factor | 0.2378 |
| Rwork | 0.237 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bi7 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.794 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.086 | 1.068 |
| Rmeas | 0.093 | 1.207 |
| Number of reflections | 87819 | 3976 |
| <I/σ(I)> | 11.3 | 1.2 |
| Completeness [%] | 99.3 | |
| Redundancy | 6.8 | 4.5 |
| CC(1/2) | 0.998 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1M sodium malonate, 12% PEG3350 |
