6VCQ

Crystal structure of E.coli RppH in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	60.671, 60.671, 74.265
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.180 - 1.600
R-factor	0.1748
Rwork	0.173
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4s2y
RMSD bond length	0.006
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.180	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	39944	6148
<I/σ(I)>	14.4
Completeness [%]	99.1
Redundancy	3.8
CC(1/2)	0.999	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.4 M (NH4)2SO4 and 10% (v/v) PEG4000