6VBY

Cinnamate 4-hydroxylase (C4H1) from Sorghum bicolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	132.278, 132.278, 79.431
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.275 - 1.700
R-factor	0.1988
Rwork	0.199
R-free	0.21480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r1z
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.275	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	87984	8712
<I/σ(I)>	4.75	1.12
Completeness [%]	99.9
Redundancy	18.6
CC(1/2)	0.995	0.462

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M HEPES, pH 7.5, 12% (v/v) isopropanol and 14% (w/v) PEG 2,000