6VAT

Structure of the periplasmic domain of YejM from Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	80
Detector technology	CMOS
Collection date	2016-06-07
Detector	RDI CMOS_8M
Wavelength(s)	1.0001
Spacegroup name	P 32 2 1
Unit cell lengths	113.799, 113.799, 299.777
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.650 - 2.350
R-factor	0.1988
Rwork	0.196
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i5f
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.650	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.211	1.987
Rpim	0.105	1.448
Number of reflections	94515	4528
<I/σ(I)>	11.1
Completeness [%]	99.9
Redundancy	9.6
CC(1/2)	0.998	0.199

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES pH 6.8 13 % PEG 3350