6VA5
Tudor Domain of Tumor suppressor p53BP1 with MFP-4184
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.678, 44.822, 65.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 1.280 |
| R-factor | 0.1743 |
| Rwork | 0.174 |
| R-free | 0.18490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rg2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.396 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.300 |
| High resolution limit [Å] | 1.280 | 3.470 | 1.280 |
| Rmerge | 0.101 | 0.055 | 0.818 |
| Rmeas | 0.110 | 0.060 | 0.919 |
| Rpim | 0.042 | 0.024 | 0.409 |
| Total number of observations | 214888 | ||
| Number of reflections | 33389 | 1829 | 1646 |
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 99.9 | 99.9 | 99.9 |
| Redundancy | 6.4 | 6.4 | 4.6 |
| CC(1/2) | 0.997 | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 2M ammonium sulfate, 0.1 M HEPES pH 7.5, 2% PEG 400 |
