6V9T
Tudor domain of TDRD3 in complex with a small molecule
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-05-28 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | H 3 2 |
Unit cell lengths | 83.855, 83.855, 114.045 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.849 - 2.154 |
Rwork | 0.196 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3pmt |
RMSD bond length | 0.017 |
RMSD bond angle | 2.025 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.850 | 44.850 | 2.220 |
High resolution limit [Å] | 2.150 | 8.880 | 2.150 |
Rmerge | 0.104 | 0.047 | 0.982 |
Rmeas | 0.109 | 0.050 | 1.030 |
Number of reflections | 8558 | 141 | 703 |
<I/σ(I)> | 18.9 | 53.7 | 2.6 |
Completeness [%] | 99.8 | ||
Redundancy | 11.1 | 9.1 | 11.1 |
CC(1/2) | 0.998 | 0.999 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.2 M sodium citrate, 0.1 M Tris-HCl, pH 8.5 |