6V8G
GltPh mutant - Y204L A345V V366A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99997 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 109.416, 109.416, 305.227 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.380 - 3.380 |
R-factor | 0.2336 |
Rwork | 0.232 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nwx |
RMSD bond length | 0.002 |
RMSD bond angle | 0.453 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.380 | 3.496 |
High resolution limit [Å] | 3.375 | 3.375 |
Rmerge | 0.115 | |
Rmeas | 0.121 | |
Rpim | 0.036 | |
Number of reflections | 30612 | 2964 |
<I/σ(I)> | 10.87 | |
Completeness [%] | 99.5 | |
Redundancy | 10.5 | |
CC(1/2) | 0.993 | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | Potassium citrate, PEG 400 |