6V7X

Structure of a phage-encoded quorum sensing anti-activator, Aqs1 bound to LasR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	105
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 41 21 2
Unit cell lengths	104.536, 104.536, 102.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.920 - 2.900
Rwork	0.249
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ix3
RMSD bond length	0.004
RMSD bond angle	0.696
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.920	3.004
High resolution limit [Å]	2.900	2.900
Number of reflections	13116	24035
<I/σ(I)>	31.89
Completeness [%]	99.9
Redundancy	2
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6M ammonium sulfate, 0.1M MES NAOH, pH 6.5 and 10% Dioxane