6V4A
An open conformation of a Pentameic ligand-gated ion channel with additional N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2016-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 141.064, 116.028, 169.630 |
| Unit cell angles | 90.00, 109.17, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.830 |
| R-factor | 0.2574 |
| Rwork | 0.256 |
| R-free | 0.29140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fli |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.983 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.610 | 3.930 |
| High resolution limit [Å] | 3.830 | 3.830 |
| Number of reflections | 49576 | 3486 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.999 | 0.448 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.3 M NH4-Formate, 0.1 M Tris 7.5, 30% (v/v) PEG-MME 500 |
