6UZX

Crystal structure of GLUN1/GLUN2A-4M mutant ligand-binding domain in complex with glycine and UBP791

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 21 21 21
Unit cell lengths	58.987, 85.081, 120.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.150 - 2.410
R-factor	0.1941
Rwork	0.191
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nf6
RMSD bond length	0.002
RMSD bond angle	0.478
Phasing software	REFMAC
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.150	2.490
High resolution limit [Å]	2.410	2.410
Rmerge	0.156	0.668
Number of reflections	23716	1995
<I/σ(I)>	11.6
Completeness [%]	98.0
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	100 mM HEPES, pH 7.0, 75 mM sodium chloride, 18% PEG2000 MME