6UZT
Crystal Structure of RPTP alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 104.720, 112.110, 136.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yfo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.476 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 29.940 | 1.830 |
| High resolution limit [Å] | 1.800 | 9.860 | 1.800 |
| Rmerge | 0.086 | 0.032 | 0.877 |
| Rmeas | 0.094 | 0.035 | 0.966 |
| Rpim | 0.037 | 0.014 | 0.401 |
| Total number of observations | 5926 | 41573 | |
| Number of reflections | 148737 | 1002 | 7290 |
| <I/σ(I)> | 10.6 | 25.3 | 1.9 |
| Completeness [%] | 100.0 | 96.7 | 100 |
| Redundancy | 6.2 | 5.9 | 5.7 |
| CC(1/2) | 0.998 | 0.999 | 0.751 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | MES, PEG 20000, DTT |
