6UXL

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Forrest complexed with PLP-Glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-03-31
Detector	RDI CMOS_8M
Wavelength(s)	1.00
Spacegroup name	P 2 21 21
Unit cell lengths	55.727, 127.445, 127.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.412 - 2.350
R-factor	0.2121
Rwork	0.210
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxh
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.412	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.151	2.231
Rmeas	0.163	2.421
Rpim	0.061	0.933
Total number of observations		25177
Number of reflections	38807	3772
<I/σ(I)>	11.2	0.8
Completeness [%]	100.0	100
Redundancy	7.1	6.7
CC(1/2)	0.998	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.18 M trimethylamine N-oxide, 0.1 M Tris pH 8.5, 22.5% PEG MME 2000