6UXI

Structure of serine hydroxymethyltransferase 8 from Glycine max cultivar Essex complexed with PLP-Glycine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 2 21 21
Unit cell lengths	56.567, 124.547, 129.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.839 - 2.100
R-factor	0.1862
Rwork	0.184
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uxh
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.840	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.144	1.828
Rmeas	0.157	1.986
Rpim	0.061	0.767
Total number of observations	353156	28628
Number of reflections	54101	4371
<I/σ(I)>	10.5	1.1
Completeness [%]	99.9	100
Redundancy	6.5	6.5
CC(1/2)	0.997	0.311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.19 M trimethylamine N-oxide, 0.1 M Tris (pH 8.5), 21% (w/v) PEG MME 2000, 20 mM glycine