6UWK

Engineered variant of I-OnuI meganuclease with improved stability and fully altered specificity targeting human chromosome 11 trans integration site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	108
Detector technology	CCD
Collection date	2019-02-14
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	38.370, 62.635, 160.199
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.524 - 2.533
R-factor	0.2389
Rwork	0.233
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qqy
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.620
High resolution limit [Å]	2.530	5.450	2.530
Rmerge	0.071	0.037	0.335
Rmeas	0.075	0.039	0.356
Rpim	0.022	0.011	0.116
Total number of observations	150302
Number of reflections	13421	1475	1212
<I/σ(I)>	10.8
Completeness [%]	99.0	98.9	91.7
Redundancy	11.2	12	8.7
CC(1/2)		1.000	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100mM sodium acetate pH 6.0, 200mM calcium acetate, 35% PEG 400