6UTA

Crystal structure of Z004 iGL Fab in complex with ZIKV EDIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 21 2
Unit cell lengths	85.907, 85.907, 327.458
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.420 - 3.100
R-factor	0.2751
Rwork	0.274
R-free	0.29120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vic
RMSD bond length	0.013
RMSD bond angle	1.571
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.420	3.210
High resolution limit [Å]	3.100	3.100
Rpim	0.088	0.491
Number of reflections	21992	1620
<I/σ(I)>	9.1	1.7
Completeness [%]	93.6	71.4
Redundancy	12.4
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	% w/v tryptone, 0.001 M sodium azide, 0.05 M HEPES sodium pH 7.0, 20% w/v polyethylene glycol 3,350