6UQR
Complex of IgE and Ligelizumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.960, 103.180, 124.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.954 - 3.650 |
| R-factor | 0.290662214839 |
| Rwork | 0.290 |
| R-free | 0.29528 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y7q 4f9p |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 3.700 |
| High resolution limit [Å] | 3.650 | 3.650 |
| Rmeas | 0.330 | |
| Number of reflections | 11282 | 430 |
| <I/σ(I)> | 8.4 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 10.9 |
| CC(1/2) | 1.000 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 287.15 | 0.2M Na Thiocyanate pH 6.9, 20% PEG3350, 10 mM Spermidine |
