6UQQ
Crystal Structure of GTPase Domain of Human Septin 7 / Septin 3 T282Y Heterocomplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.968630 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.390, 85.526, 152.051 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 61.381 - 2.750 |
| R-factor | 0.1999 |
| Rwork | 0.197 |
| R-free | 0.25550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qnr 4z54 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.559 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 61.381 | 61.380 | 2.870 |
| High resolution limit [Å] | 2.750 | 9.530 | 2.750 |
| Rmerge | 0.169 | 0.056 | 0.922 |
| Rmeas | 0.197 | 0.066 | 1.080 |
| Rpim | 0.098 | 0.034 | 0.548 |
| Total number of observations | 3302 | 17068 | |
| Number of reflections | 38827 | 939 | 4789 |
| <I/σ(I)> | 5.7 | 16.5 | 1.3 |
| Completeness [%] | 94.8 | 91.6 | 96.4 |
| Redundancy | 3.6 | 3.5 | 3.6 |
| CC(1/2) | 0.988 | 0.994 | 0.604 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 10% PEG 4000, 20% glycerol, 0.1 M MES/imidazole pH 6.5 and 20mM of each sodium formate, ammonium acetate, trisodium citrate, sodium potassium l-tartrate, sodium oxamate |
