6UPQ

Crystal Structure of GTPase Domain of Human Septin 2 / Septin 11 Heterocomplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96863
Spacegroup name	P 1 21 1
Unit cell lengths	56.654, 81.859, 68.221
Unit cell angles	90.00, 97.13, 90.00

Refinement procedure

Resolution	56.216 - 1.860
R-factor	0.1882
Rwork	0.187
R-free	0.21780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6upa
Data reduction software	xia2
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.700	67.700	1.910
High resolution limit [Å]	1.860	8.320	1.860
Rmerge	0.192	0.060	3.126
Rmeas	0.208	0.065	3.387
Rpim	0.080	0.026	1.292
Total number of observations	347126	3914	25676
Number of reflections	51728	611	3798
<I/σ(I)>	5.8	15.5	0.9
Completeness [%]	99.6	99.9	98.9
Redundancy	6.7	6.4	6.8
CC(1/2)	0.973	0.996	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	100mM MES/imidazole pH 6.5, 10% PEG 20000, 20% PEG 550 MME and 30mM of each sodium nitrate, disodium hydrogen phosphate and ammonium sulfate