6UM8
HIV Integrase in complex with Compound-14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 164.626, 164.626, 164.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 116.410 - 2.330 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ncj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 164.510 | 164.510 |
| High resolution limit [Å] | 2.330 | 8.970 |
| Number of reflections | 33296 | 18666 |
| <I/σ(I)> | 42.2 | 79.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 38.5 | 29.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.0 M ammonium sulfate and 100 mM citric acid pH 3.5 |
