6UKV
STING C-terminal Domain Complexed with Non-cyclic Dinucleotide Compound 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.510, 109.390, 59.150 |
| Unit cell angles | 90.00, 95.69, 90.00 |
Refinement procedure
| Resolution | 26.790 - 1.830 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6UKM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data scaling software | Aimless (0.7.1) |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.855 | 1.862 |
| High resolution limit [Å] | 1.830 | 1.831 |
| Rmerge | 0.052 | 0.528 |
| Rmeas | 0.061 | 0.521 |
| Rpim | 0.033 | 0.280 |
| Total number of observations | 19958 | |
| Number of reflections | 40497 | 5874 |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.999 | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | 25% PEG 6000, 100 mM Tris, 200 mM NaCl, 2 mM DTT |
