6UK1
Crystal structure of nucleotide-binding domain 2 (NBD2) of the human Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.063, 60.132, 105.160 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 64.414 - 2.693 |
| R-factor | 0.2749 |
| Rwork | 0.273 |
| R-free | 0.30360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gd7 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 5.980 |
| High resolution limit [Å] | 2.693 | 2.700 |
| Rmeas | 0.116 | 0.116 |
| Rpim | 0.050 | 0.050 |
| Number of reflections | 32360 | 28962 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 277 | 24% PEG 8000, 100 mM Li2SO4, 10mM NaBr, 100 mM Bis-Tris Propane pH 6.0 |
