6UIT

HIV-1 wild-type reverse transcriptase-DNA complex with dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97395
Spacegroup name	C 2 2 21
Unit cell lengths	167.525, 167.790, 102.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.095 - 2.806
R-factor	0.189329793653
Rwork	0.185
R-free	0.25602
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kk1
RMSD bond length	0.010
RMSD bond angle	1.231
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.081	0.627
Number of reflections	35132	3187
<I/σ(I)>	19.3
Completeness [%]	99.6
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	2% PEG 4000, 100mM MES pH 6, 10mM magnesium sulfate