6UIQ

Crystal structure of wild-type human phosphoglucomutase 1 in complex with Glucose-6-Phosphate

Replaces:  6BJ0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-09-23
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 41 21 2
Unit cell lengths	171.874, 171.874, 99.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.810 - 2.300
R-factor	0.170935744974
Rwork	0.168
R-free	0.22252
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5epc
RMSD bond length	0.007
RMSD bond angle	0.893
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.820	60.820	2.360
High resolution limit [Å]	2.300	10.540	2.300
Rmerge	0.160	0.035	1.774
Rmeas	0.167	0.036	1.868
Rpim	0.045	0.010	0.573
Total number of observations	878402	10241	37536
Number of reflections	64976	809	3790
<I/σ(I)>	14.8	51.5	1.3
Completeness [%]	97.8	99.4	82.6
Redundancy	13.5	12.7	9.9
CC(1/2)	0.998	0.999	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	0.2 M Sodium tartrate dibasic dihydrate, 20% w/v Polyethylene glycol 3,350